| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Yining Lei, Manqin Li, Xinran Liu, Lu Zhang, Ruyi Zhang, Fei Ca. Nerolidol rescues hippocampal injury of diabetic rats through inhibiting NLRP3 inflammasome and regulation of MAPK/AKT pathway. BioFactors (Oxford, England). 2024-04-16. PMID:38624190. |
western blot was used to detect apoptosis (bcl-2, bax, and cleaved-caspase-3); synapses (postsynaptic densifying protein 95, syn1, and synaptophysin); mitochondria (drp1, opa1, mfn1, and mfn2); er (grp78, atf6, chop, and caspase-12); nlrp3 inflammasome (nlrp3, asc, and caspase-1); inflammatory cytokines (il-18, il-1β, and tnf-α); akt (p-akt); and mitogen-activated protein kinase (mapk) pathway (p-erk, p-p38, and p-jnk) related protein expression. |
2024-04-16 |
2024-04-18 |
rat |
| Yanyan Wu, Chenhang Yao, Lan Zhang, Guoqing W. Sophora flavescens alcohol extract ameliorates insomnia and promotes PI3K/AKT/BDNF signaling transduction in insomnia model rats. Neuroreport. 2024-02-26. PMID:38407863. |
the levels of monoamine neurotransmitters in rat hypothalamus were determined using elisa, and the transduction of the phosphatidylinositol 3-kinase (pi3k)/protein kinase b (akt)/brain-derived neurotrophic factor (bdnf) signaling in the brain tissues of rats was examined by western blot. |
2024-02-26 |
2024-02-28 |
rat |
| Nan Nan, Xiao-Li DU, Miao Chen, Jia-Qi Lu. [Qirong Tablets inhibits apoptosis of ovarian granulosa cells via PI3K/Akt/ HIF-1 signaling pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 48. issue 17. 2023-10-06. PMID:37802816. |
terminal deoxynucleotidyl transferase-mediated dutp nick end labeling(tunel) assay was used to detect the apoptosis of granulosa cells, and western blot to determine the expression levels of b-cell lymphoma-2(bcl-2), bcl-2-associated x protein(bax), caspase-3, pi3k, akt, and hif-1. |
2023-10-06 |
2023-10-15 |
mouse |
| Lei Wu, Siwei Wei, Dongjie Pei, Yiyi Yao, Zhen Xiang, Eryou Yu, Zheng Chen, Zhen Du, Shuangquan Q. Activation of the Akt Attenuates Ropivacaine-Induced Myelination Impairment in Spinal Cord and Sensory Dysfunction in Neonatal Rats. Molecular neurobiology. 2023-07-31. PMID:37523045. |
western blot analysis and immunostaining was performed to detect the expression changes of p-akt, akt, myelin gene regulatory factor (myrf) and myelin basic protein (mbp) in the spinal cord treated with different concentrations of ropivacaine. |
2023-07-31 |
2023-08-14 |
rat |
| Liangzi Cao, Hao Li, Xiaofang Liu, Yubang Wang, Bowen Zheng, Chengzhong Xing, Naijin Zhang, Jingwei Li. Expression and regulatory network of E3 ubiquitin ligase NEDD4 family in cancers. BMC cancer. vol 23. issue 1. 2023-06-08. PMID:37291499. |
nedd4 family members interacted proteins were enriched in various pathways including p53, akt, apoptosis and autophagy, which were confirmed by further western blot and flow cytometric analysis in a549 and h1299 lung cancer cells. |
2023-06-08 |
2023-08-14 |
Not clear |
| Yuanming Lu, Wanshi Wang, Yanping Ma, Zilian Fan, Lan Xiong, Junhao Zhao, Yongwen He, Chao Li, Anjie Wang, Nanping Xiao, Tianxun Wan. miR-10a induces inflammatory responses in epileptic hippocampal neurons of rats via PI3K/Akt/mTOR signaling pathway. Neuroreport. vol 34. issue 10. 2023-06-04. PMID:37270844. |
the hippocampal neurons were transfected with mir-10a mimics, and transcript levels of mir-10a, pi3k, akt and mtor were detected by quantitative reverse transcription-pcr, and pi3k, mtor, akt, tnf-α, il-1β, il-6 protein expression levels were detected by western blot. |
2023-06-04 |
2023-08-14 |
rat |
| Xueyao Yin, Zhiye Xu, Xinxin Zhang, Jiahua Wu, Weina L. Deficiency of lipopolysaccharide binding protein facilitates adipose browning, glucose uptake and oxygen consumption in mouse embryonic fibroblasts via activating PI3K/Akt/mTOR pathway and inhibiting autophagy. Cell cycle (Georgetown, Tex.). 2023-01-30. PMID:36710409. |
the expression levels of lbp, inflammatory markers , brown fat markers, lipid metabolism marker, autophagy markers, insulin signaling-related molecules , p-mtor, mtor, p-akt, akt, p-pi3k, and pi3k were quantified or determined by western blot, qrt-pcr, and immunofluorescence assay. |
2023-01-30 |
2023-08-14 |
mouse |
| Yangjie Li, Zhengdong Wang, Jing Li, Yang Yu, Yuan Wang, Xiaoxia Jin, Yun Dong, Qingsong Liu, Xiaoxu Duan, Nan Ya. Sodium Butyrate Ameliorates Fluorosis-Induced Neurotoxicity by Regulating Hippocampal Glycolysis In Vivo. Biological trace element research. 2023-01-29. PMID:36710293. |
western blot results showed that the abundance of malate dehydrogenase 2 (mdh2) and pyruvate dehydrogenase (pdh) in the hippocampus of fluorosis mice was increased, the abundance of pyruvate kinase m (pkm), lactate dehydrogenase (ldh), hexokinase (hk), phosphatidylinositol 3-kinase (pi3k), phosphorylated akt (p-akt), and hypoxia-inducible factor 1α (hif-1α) was inhibited, and the content of lactate and atp was decreased. |
2023-01-29 |
2023-08-14 |
mouse |
| Mai Yin Cui, Yan Qiong Fu, Zhuo Li Li, Yu Zheng, Yi Yu, Chan Zhang, Yun Qi Zhang, Bing Rui Gao, Wu Yue Chen, Yun-Lyul Lee, Moo-Ho Won, Min Liao, Yong Jian, Bai Hui Che. Neuregulin-1/PI3K signaling effects on oligodendrocyte proliferation, remyelination and behaviors deficit in a male mouse model of ischemic stroke. Experimental neurology. 2023-01-23. PMID:36690057. |
in addition, the effect of nrg1 on neuronal damage/death (cresyl violet, cv), neuronal nuclei (neun), nestin, doublecortin (dcx), myelin basic protein (mbp), non-phosphorylated neurofilaments (smi-32), adenomatous polyposis coli (apc), erythroblastic leukemia viral oncogene homolog (erbb) 2, 4 and serine-threonine protein kinase (akt) in cortex were evaluated using immunohistochemistry, immunofluorescence and western blot. |
2023-01-23 |
2023-08-14 |
mouse |
| Wei Sun, Nai-Dan Zhang, Tong Zhang, Yan-Nan Li, Hui Xue, Jing-Long Cao, Wen-Shuang Hou, Jian Liu, Ying Wang, Cheng-Hao Ji. Cyanidin-3-O-Glucoside Induces the Apoptosis of Human Gastric Cancer MKN-45 Cells through ROS-Mediated Signaling Pathways. Molecules (Basel, Switzerland). vol 28. issue 2. 2023-01-21. PMID:36677726. |
western blot and flow cytometry results showed that c3g regulated the reduction of mitochondrial membrane potential and arrested the cell cycle in the g2/m phase through the akt signaling pathway, causing the cells to undergo apoptosis. |
2023-01-21 |
2023-08-14 |
human |
| Yong-Fang Yang, Shan-Xin Peng, Kai-Yue Wang, Chong-Tian Zhu, Hua-Sheng Zhong, Feng-Yuan Ch. [Effect of Mailuo Shutong Pills in treatment of ischemic stroke based on network pharmacological analysis and experimental verification]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 23. 2023-01-06. PMID:36604893. |
after the administration, cerebral infarction volume was detected by 2,3,5-triphenyltetrazoliumchloride(ttc) staining, and western blot was performed to determine the levels of phosphatidylinositol 3-kinase(pi3 k), protein kinase b(akt), nuclear factor-κb(nf-κb) and their phosphorylated proteins. |
2023-01-06 |
2023-08-14 |
mouse |
| Yael Yuhas, Shai Ashkenazi, Eva Berent, Abraham Weizma. Clozapine Suppresses the Gene Expression and the Production of Cytokines and Up-Regulates Cyclooxygenase 2 mRNA in Human Astroglial Cells. Brain sciences. vol 12. issue 12. 2022-12-23. PMID:36552163. |
clozapine inhibited akt phosphorylation induced by the cytokine mix at 10 min and 40 min, as assessed by western blot analysis with anti-pt308akt antibody. |
2022-12-23 |
2023-08-14 |
human |
| Shuancheng Zhang, Yucong Ma, Qianqian Zuo, Mengrui Liu, Zhifang Yu, Ying Sun, Cuimiao Song, Hongyan Sun, Guimin Hao, Bulang Gao, Huilan Du, Jingwei Che. Repeated controlled ovarian stimulation-induced ovarian and uterine damage in mice through the PI3K/AKT signaling pathway. Human cell. 2022-11-28. PMID:36441500. |
the morphology of the ovary and endometrium, ovarian apoptosis, and expressions of the vascular endothelial growth factor (vegf), leukemia inhibitory factor (lif), pi3k, akt, bax, and bcl-2 in the ovarian and uterine tissues were tested by hematoxylin-eosin (he) staining, immunohistochemistry, and western blot. |
2022-11-28 |
2023-08-14 |
mouse |
| Yupeng Chen, Lin Wu, Min Ba. MiR-485-5p Suppress the Malignant Characteristics of the Lung Adenocarcinoma via Targeting NADPH Quinone Oxidoreductase-1 to Inhibit the PI3K/Akt. Molecular biotechnology. 2022-10-11. PMID:36219369. |
western blot experiments were used to examine the relative protein expression of matrix metallopeptidase 2 and matrix metallopeptidase 9, as well as the phosphorylation of phosphoinositide 3-kinase (pi3k) and protein kinase b (akt) in luad cells. |
2022-10-11 |
2023-08-14 |
Not clear |
| Sang-Seo Park, Tae-Woon Kim, Bo-Kyun Kim, Seong-Hyun Kim, Jong-Suk Park, Mal-Soon Shi. The effects of exercise and diet on mental status, insulin signaling pathway, and microbiome in obese mice. Journal of exercise rehabilitation. vol 18. issue 3. 2022-07-18. PMID:35846233. |
immunohistochemistry for tryptophan hydroxylase (tph) in the dorsal raphe, western blot analysis for phosphorylated protein kinase b (p-atk), total akt (t-akt), phosphorylated phosphoinositide 3-kinase (p-pi3k), and total pi3k (t-pi3k) in the hippocampus were performed. |
2022-07-18 |
2023-08-14 |
mouse |
| Li-Ping Huang, Xiao-Qin Zhong, Xin-Yu Zhou, Min-Qi Deng, Man-Jing Wu, Min-Zhen Den. [Galangin alleviates learning and memory impairments in APP/PS1 double- transgenic mice by regulating Akt/MEF2D/Beclin-1 signaling pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 10. 2022-06-19. PMID:35718493. |
the pathological changes in hippocampus were observed after he staining, and the expression of akt, mef2 d, and beclin-1 in hippocampus were assayed by western blot. |
2022-06-19 |
2023-08-14 |
mouse |
| Quan-Cao Zhan, Yu-Zhe Liu, Yu-Meng Xi, Yan Zeng, Jia-Yu Tian, Li Liu, Dong Li, Yamahara Johji, Jian-Wei Wan. [6-Gingerol ameliorates adipose tissue insulin resistance in aging rats]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 6. 2022-03-29. PMID:35347963. |
the gene and protein expression levels of adiponectin receptor 1(adipor1), amp-activated protein kinase α(ampkα), phosphorylated ampk(p-ampkα~(thr172)), peroxisome proliferator-activated receptor-γ coactivator-1α(pgc-1α), phosphatidylinositol 3-kinase(pi3 k), protein kinase b(akt), phosphorylated akt(p-akt~(ser473)), tumor necrosis factor-α(tnf-α), c-jun n-terminal kinase 1/2(jnk1/2), phosphorylated jnk1/2(p-jnk~(thr183/tyr185)), interleukin-1β(il-1β), and interleukin-6(il-6) in adiponectin(apn), insulin, and inflammatory factor signaling pathways were detected by western blot and real-time rcr, respectively. |
2022-03-29 |
2023-08-13 |
rat |
| Jian Wang, Yongzhen Huang, Jiawei Xu, Binglin Yue, Yifan Wen, Xiao Wang, Chuzhao Lei, Hong Che. PLAG1 promotes proliferation and inhibits apoptosis of bovine primary myoblasts through the PI3K-Akt signaling pathway. Journal of animal science. 2022-03-24. PMID:35325183. |
chromatin immunoprecipitation sequencing (chip-seq) and western blot showed plag1 upregulated phosphorylated (p)-pi3k, pi3k, p-akt, akt, cyclin d1, and cdk2 and inhibited the expression of p21 and p27 to enhance myoblast proliferation, and increased expression of bcl-2, and bcl-xl to inhibit apoptosis. |
2022-03-24 |
2023-08-13 |
cattle |
| Mădălina Dumitrescu, Alina Constantin, Andreea Miruna Nemecz, Emanuel Drăgan, Lucia Doina Popov, Gabriela Tank. Hypertension induces compensatory left ventricular hypertrophy by a mechanism involving gap junction lateralization and overexpression of CD36, PKC and MMP-2. Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie. vol 62. issue 3. 2022-03-09. PMID:35263399. |
as compared to age-matched control hamsters, the hypertrophied left ventricles in l-name-induced ht hamsters exhibited the following structural and molecular changes: (i) accumulation of lipid droplets (lds) within cardiomyocytes and relocation of gap junctions to the lateral membrane of cardiomyocytes or close to mitochondria (revealed by electron microscopy); (ii) overexpression of the cluster of differentiation 36 (cd36) fatty acid transporter, protein kinase c (pkc), and matrix metalloproteinase-2 (mmp-2), enhanced activation of the phosphoinositide 3-kinase (pi3k)/protein kinase b (akt) pathway, and unchanged expression of the connexin 43 (cx43) and n-cadherin junctional proteins (assessed by western blot); (iii) increased protein carbonyl content, assessed with a 2,4-dinitrophenylhydrazine (dnph)-based spectrophotometric assay, indicative of an enhanced reactive oxygen species (ros) production; and (iv) augmented mmp-2 activity (determined by gelatin zymography). |
2022-03-09 |
2023-08-13 |
Not clear |
| Marta Gallardo-Fernández, Ana B Cerezo, Ruth Hornedo-Ortega, Ana M Troncoso, M Carmen Garcia-Parrill. Anti-VEGF Effect of Bioactive Indolic Compounds and Hydroxytyrosol Metabolites. Foods (Basel, Switzerland). vol 11. issue 4. 2022-02-25. PMID:35206003. |
experiments involved the evaluation of their potential to inhibit vegf on human umbilical vein endothelial cells (huvecs) by elisa assay and their subsequent effect on the downstream signaling pathway (plcγ1, akt, and endothelial nitric oxide synthetase (enos)) by western blot. |
2022-02-25 |
2023-08-13 |
human |