| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| b' Sabriye Karadenizli Ta\\xc5\\x9fkin, Deniz \\xc5\\x9eahin, Fazilet Dede, Zehra Seda \\xc3\\x9cnal Halbuto\\xc4\\x9fullari, Mehmet Sarihan, Sema Kurnaz \\xc3\\x96zbek, \\xc3\\x96zg\\xc3\\xbcr Do\\xc4\\x9fa \\xc3\\x96zsoy, Murat Kasap, Yusufhan Yazir, Nurbay Ate\\xc5\\x9. Endoplasmic reticulum stress produced by Thapsigargin affects the occurrence of spike-wave discharge by modulating unfolded protein response pathways and activating immune responses in a dose-dependent manner. European journal of pharmacology. 2024-04-26. PMID:38670446.' |
in cortex and thalamus tissues, grp78, erp57, gad153 protein changes (western blot), eif2ak3, xbp-1, atf6, cacna1h mrna expressions (rt-pcr), nf-κb and tnf-α levels (elisa) were measured. |
2024-04-26 |
2024-04-29 |
rat |
| Yining Lei, Manqin Li, Xinran Liu, Lu Zhang, Ruyi Zhang, Fei Ca. Nerolidol rescues hippocampal injury of diabetic rats through inhibiting NLRP3 inflammasome and regulation of MAPK/AKT pathway. BioFactors (Oxford, England). 2024-04-16. PMID:38624190. |
western blot was used to detect apoptosis (bcl-2, bax, and cleaved-caspase-3); synapses (postsynaptic densifying protein 95, syn1, and synaptophysin); mitochondria (drp1, opa1, mfn1, and mfn2); er (grp78, atf6, chop, and caspase-12); nlrp3 inflammasome (nlrp3, asc, and caspase-1); inflammatory cytokines (il-18, il-1β, and tnf-α); akt (p-akt); and mitogen-activated protein kinase (mapk) pathway (p-erk, p-p38, and p-jnk) related protein expression. |
2024-04-16 |
2024-04-18 |
rat |
| Qionghua Hu, Lingqin Qiu, Li Ge, Yi We. Sevoflurane postconditioning alleviates hypoxic-ischemic brain damage in rats by inhibiting the endoplasmic reticulum stress PERK/ATF4/CHOP pathway. Tissue & cell. vol 86. 2024-01-09. PMID:38194851. |
additionally, nissl staining assessed hippocampal ca1 area neuronal density, tunel staining evaluated hippocampal ca1 area neuronal apoptosis, and western blot determined hippocampal ca1 area protein levels, including bax, bcl-2, p-perk/perk, p-eif2/eif2, atf4, chop, grp78, bax, and bcl-2 protein levels. |
2024-01-09 |
2024-01-13 |
rat |
| Jing Zhang, Qingbo Zhao, Zhihui Xue, Siyi Zhang, Zeyu Ren, Shaokui Chen, Ao Zhou, Hongbo Chen, Yulan Li. Deoxynivalenol induces endoplasmic reticulum stress-associated apoptosis via the IRE1/JNK/CHOP pathway in porcine alveolar macrophage 3D4/21 cells. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 2023-09-22. PMID:37739053. |
our subsequent validation via qpcr and western blot analyses confirmed the attenuation of grp78 and bcl-2, coupled with the upregulation of ire1, chop, jnk, p-jnk, and bax in don-induced cells, indicating the instigation of er stress-associated apoptosis by don. |
2023-09-22 |
2023-10-07 |
Not clear |
| Meng-Yu Zuo, Tong-Juan Tang, Peng Zhou, Xiang Wang, Rui Ding, Jin-Fan Gu, Jian Chen, Liang Wang, Juan Yao, Xiang-Yang Li, Jin-Ling Huan. [Mechanism of atractylenolide Ⅲ in alleviating H9c2 cell apoptosis through ROS/GRP78/caspase-12 signaling pathway based on molecular docking]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 47. issue 16. 2022-09-01. PMID:36046873. |
this study aims to investigate the effect of atractylenolide Ⅲ(atl-Ⅲ) on hydrogen peroxide(h_2o_2)-induced endoplasmic reticulum stress and apoptosis of h9 c2 cells via the ros/grp78/caspase-12 signaling pathway.the binding activity of atl-Ⅲ to grp78 was determined by molecular docking.the result showed that atl-Ⅲ had a good binding activity to grp78, and the binding activity of atl-Ⅲ was stronger than that of its specific inhibitor.the endoplasmic reticulum stress model of h9 c2 was established by h_2o_2(100 μmol·l~(-1)) treatment.five groups were designed: blank control group, model group, and atl-Ⅲ(15, 30, and 60 μmol·l~(-1)) groups.apoptosis was detected by hoechst/pi double staining and flow cytometry.the levels of superoxide dismutase(sod), malondialdehyde(mda), and lactate dehydrogenase(ldh) were measured by colorimetry.the levels of reactive oxygen species(ros) and calcium(ca~(2+)) in cytoplasm were determined by the fluorescence probe dcfh-da and the calcium fluorescence probe flou-4, respectively.the protein levels of grp78, caspase-12, and caspase-3 were determined by western blot, and the mrna levels of grp78 and caspase-12 by rt-qpcr.n-acetyl-l-cysteine(nac) and 4-phenylbutyric acid(4-pba) were respectively used to inhibit ros and grp78, and then the mechanism of atl-Ⅲ in protecting the cells from endoplasmic reticulum stress induced by h_2o_2 were deduced.atl-Ⅲ(15, 30, and 60 μmol·l~(-1)) decreased the apoptosis rate and ros, mda, and ldh levels(p<0.01), increased the sod activity(p<0.01), and down-regulated the protein levels of grp78, caspase-12, and caspase-3 and the mrna levels of grp78 and caspase-12(p<0.05).the addition of nac decreased the apoptosis rate and ros, mda, grp78, caspase-12, and caspase-3 levels(p<0.01), while it elevated the sod level(p<0.01).the addition of 4-pba also decreased the apoptosis rate and the levels of grp78, caspase-12, caspase-3, and ca~(2+)(p<0.01).the effect of inhibitors were consistent with that of atl-Ⅲ.in conclusion, atl-Ⅲ can protect h9 c2 cardiomyocytes by regulating ros/grp78/caspase-12 signaling pathway to inhibit h_2o_2-induced endoplasmic reticulum stress and apoptosis. |
2022-09-01 |
2023-08-14 |
Not clear |
| Zhang Bao, Yin Jin. Brominated flame retardant TBPH induced oxidative damage and reduced the expression of memory-related proteins in mice, with no discernable impairment of learning and memory. Human & experimental toxicology. vol 40. issue 12_suppl. 2021-12-08. PMID:34784780. |
western blot was used to detect the expression of glucose-regulated protein 78-kda (grp78), pkr-like er kinase (perk), and c/ebp homologous protein (chop) in the hippocampus. |
2021-12-08 |
2023-08-13 |
mouse |
| Qiang Zuo, Yunsheng Ou, Shenxi Zhong, Haoyang Yu, Fangbiao Zhan, Muzi Zhan. Targeting GRP78 enhances the sensitivity of HOS osteosarcoma cells to pyropheophorbide-α methyl ester-mediated photodynamic therapy via the Wnt/β-catenin signaling pathway. Acta biochimica et biophysica Sinica. vol 53. issue 10. 2021-10-15. PMID:34494093. |
apoptosis and reactive oxygen species (ros) levels were detected by hoechst staining and flow cytometry, cell viability was detected by cell counting kit-8 assay, grp78 protein fluorescence intensity was detected by immunofluorescence, and apoptosis-related proteins, cell proliferation-related proteins, and wnt pathway-related proteins were detected by western blot. |
2021-10-15 |
2023-08-13 |
human |
| Man-Lin Guo, Xiang-Yu Ma, Yu-Qing Gong, Meng-Lin Feng, Yu-Wan Zhao, Leng-Xin Dua. [Protective effect of ethyl acetate extract from Bidens bipinnata on hepatocyte damage induced by endoplasmic reticulum stress]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 46. issue 15. 2021-09-03. PMID:34472265. |
methyl thiazolyl tetrazolium(mtt) colorimetric assay was used to investigate the survival rate of ethyl acetate extract from b. bipinnata in l02 cells injury induced by endoplasmic reticulum stress; the protein expressions of endoplasmic reticulum stress-related molecule glucose regulated protein 78(grp78), pkr-like er kinase(perk), eukaryotic initiation factor-2(eif2α), activating transcription factor 4(atf4), c/ebp homologous protein(chop), b-cell cll/lymphoma 2(bcl-2), bal-2 associated x apoptosis regulator(bax) were examined by wes-tern blot. |
2021-09-03 |
2023-08-13 |
Not clear |
| Ye Xiong, Dan-Qing Xin, Quan Hu, Ling-Xiao Wang, Jie Qiu, Hong-Tao Yuan, Xi-Li Chu, De-Xiang Liu, Gang Li, Zhen Wan. Neuroprotective mechanism of L-cysteine after subarachnoid hemorrhage. Neural regeneration research. vol 15. issue 10. 2020-10-09. PMID:32246641. |
western blot assays and immunohistochemistry were used to detect the protein levels of chop and grp78 in the prefrontal cortex and the number of chop- and grp78-positive cells. |
2020-10-09 |
2023-08-13 |
rat |
| Lina Zhao, Meili Zhai, Xu Yang, Hongjie Guo, Ying Cao, Donghui Wang, Ping Li, Chong Li. Dexmedetomidine attenuates neuronal injury after spinal cord ischaemia-reperfusion injury by targeting the CNPY2-endoplasmic reticulum stress signalling. Journal of cellular and molecular medicine. vol 23. issue 12. 2020-09-22. PMID:31625681. |
to determine whether the endoplasmic reticulum apoptosis pathway cnpy2-perk was involved in the neuroprotective mechanism of dex, the expression levels of related proteins (cnpy2, grp78, perk, chop, caspase-12, caspase-9 and caspase-3) were detected by western blot analysis and rt-pcr. |
2020-09-22 |
2023-08-13 |
rat |
| Xiao-le Gao, Leng-Xin Duan, Ke-Ke Qiu, Man-Lin Guo, Ye-Lin Jiao, Dong-Mei Wan. [Effect and mechanism of Bidens pilosa decoction on non-alcoholic fatty liver induced by high fat and high glucose in mice]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 45. issue 16. 2020-09-17. PMID:32893589. |
the results showed that compared with the model group, blood lipid and blood glucose levels of each treatment group were significantly lower(p<0.05); he staining results showed that liver pathological damage in each treatment group was significantly improved; oil red o staining results showed fat distribution in each treatment group significantly reduced(p<0.01); immunohistochemical staining showed that glucose regulated the protein expression of protein 78(grp78) in liver tissues of each treatment group was also significantly reduced(p<0.01); western blot results showed that endoplasmic reticulum stress signal pathway-related factors grp78, phosphorylated-protein kinase r-like er kinase(p-perk), eukaryotic translation-initiation factor 2α(eif2α), activating transcription factor 4(atf4), c/ebp homologous protein(chop), inositol requiring 1α(ire1α), and cleaved-cysteinyl aspartate specific proteinase 12(cleaved-caspase-12) were significantly reduced(p<0.01). |
2020-09-17 |
2023-08-13 |
mouse |
| Zi-Jian Yang, Jun-Yao Huang, Ye-Fei Gao, Jun-Yao Huang, Wen-Ji Huang, Jun-Yao Huang, Shi-Lei Xu, Jun-Yao Huang, Jing-Jing Jin, Jun-Yao Huang, Ye-Dong Wan, Jun-Yao Huang, Ming Yan, Jun-Yao Huang, Hong-Jiao Mao, Jun-Yao Huang, Yun Zhang, Jun-Yao Huan. [Effects of bergapten on damages of osteocytes MLO-Y4 induced by TCP wear particles and its mechanism]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology. vol 35. issue 6. 2020-04-14. PMID:32239867. |
mtt assay and calcein-am staining were used to determine the viability of mlo-y4 cells; hoechst 33342 staining and the flow cytometry were applied to detect the apoptosis of mlo-y4; real-time pcr was performed to examine the mrna levels of dentin matrix protein1 (dmp-1), sclerostin (sost) and fibroblast growth factor23 (fgf23); western blot was performed to examine protein expressions of glucose-regulated protein 78 (grp78), protein kinase r-like er kinase (perk) phospho-perk (p-perk), eukaryotic initiation factor 2α (eif2α), phospho-eif2α (p-eif2α), activating transcription factor 4 (aft4), c/ebp homologous protein (chop) and caspase-3 in mlo-y4 cells. |
2020-04-14 |
2023-08-13 |
Not clear |
| Yongxiang Wang, Chunyan Wan. microRNA-211-3p has a Role in the Effects of Lipopolysaccharide on Endoplasmic Reticulum Stress in Cultured Human Skin Fibroblasts. Medical science monitor basic research. vol 25. 2020-01-24. PMID:31221950. |
protein expression levels of the transcription factors grp78, chop, p-jnk, and the endoplasmic reticulum stress apoptosis proteins, caspase-12 and bcl-2, were determined by western blot. |
2020-01-24 |
2023-08-13 |
human |
| Elisa Landucci, Irene L Llorente, Berta Anuncibay-Soto, Domenico E Pellegrini-Giampietro, Arsenio Fernández-Lópe. Bicuculline Reverts the Neuroprotective Effects of Meloxicam in an Oxygen and Glucose Deprivation (OGD) Model of Organotypic Hippocampal Slice Cultures. Neuroscience. vol 386. 2019-03-11. PMID:29949743. |
western blot assays were performed to measure levels of cleaved and non-cleaved caspase-3 to quantify apoptosis, while levels of grp78, grp94 and phosphorylated eif2α were used to detect unfolded protein response (upr). |
2019-03-11 |
2023-08-13 |
rat |
| Renata Minuzzo Hartmann, Francielli Licks, Elizângela Gonçalves Schemitt, Josieli Raskopf Colares, Mariana do Couto Soares, Gilmara Pandolfo Zabot, Henrique Sarubbi Fillmann, Norma Possa Marron. Protective effect of glutamine on the main and adjacent organs damaged by ischemia-reperfusion in rats. Protoplasma. vol 254. issue 6. 2018-06-25. PMID:28382390. |
blood and tissue of were collected for aspartate aminotransferase (ast) and alanine aminotransferase (alt) levels, histopathological analysis, immunohistochemistry of il-1β and tnf-α, thiobarbituric acid reactive substance (tbars) and nitric oxide, nrf2/keap1, superoxide dismutase (sod), nadph quinone oxidoreductase1 (nqo1), inducible nitric oxide synthase (inos), heat shock protein (hsp70), glucose-regulated protein 78 (grp78), and activating transcription factor 6 (atf-6) by western blot. |
2018-06-25 |
2023-08-13 |
rat |
| Guangyu Jiao, Liying Hao, Mengmeng Wang, Bin Zhong, Miao Yu, Shuang Zhao, Pingping Wang, Rui Feng, Shutao Tan, Liu Che. Upregulation of endoplasmic reticulum stress is associated with diaphragm contractile dysfunction in a rat model of sepsis. Molecular medicine reports. vol 15. issue 1. 2017-03-30. PMID:27959404. |
the mrna and protein expression of glucose‑regulated protein 78 kda (grp78), glucose-regulated protein 94 kda (grp94), c/ebp homologous protein (chop), endoplasmic reticulum protein 44 (erp44), protein disulfide-isomerase like protein (erp57) and protein disulfide isomerase family a member 4 (erp72) in diaphragm muscles were measured using reverse transcription‑quantitative polymerase chain reaction and western blot analysis. |
2017-03-30 |
2023-08-13 |
rat |
| Xueying Feng, Dongchuan Zhang, Qingjin Gong, Zhiyong Zhang, Li Qua. Expression of Glucose-Regulated Protein 78 and miR-199a in Rat Brain After Fatal Ligature Strangulation. The American journal of forensic medicine and pathology. vol 38. issue 1. 2017-03-29. PMID:28072596. |
the expressions of grp78 and mir-199a in rat cortex, hippocampi, and midbrain were measured by immunohistochemistry and western blot analysis in a rat model of ligature strangulation. |
2017-03-29 |
2023-08-13 |
rat |
| Zhangyuan Xiong, Rong Jiang, Xiangzhu Li, Yanna Liu, Fengjin Gu. Different Roles of GRP78 on Cell Proliferation and Apoptosis in Cartilage Development. International journal of molecular sciences. vol 16. issue 9. 2016-06-17. PMID:26370957. |
furthermore, overexpression of grp78 inhibited er stress-mediated apoptosis and then reduced apoptosis in chondrogenesis induced by bmp2, as assayed by cleaved caspase3, caspase12, c/ebp homologous protein (chop/ddit3/gadd153), p-jnk (phosphorylated c-jun n-terminal kinase) expression during the course of chondrocyte differentiation by western blot. |
2016-06-17 |
2023-08-13 |
Not clear |
| Pingan Peng, Qian Ma, Le Wang, Ou Zhang, Hongya Han, Xiaoli Liu, Yujie Zhou, Yingxin Zha. Preconditioning With Tauroursodeoxycholic Acid Protects Against Contrast-Induced HK-2 Cell Apoptosis by Inhibiting Endoplasmic Reticulum Stress. Angiology. vol 66. issue 10. 2016-01-11. PMID:25818106. |
cell viability test, hoechst 33258 staining, and flow cytometry were used to detect meglumine diatrizoate-induced cell apoptosis, while real-time polymerase chain reaction and western blot analysis were used to measure the expressions of ers markers of glucose-regulated protein 78 (grp78), activating transcription factor 4 (atf4), and the apoptosis-related marker of caspase 12. |
2016-01-11 |
2023-08-13 |
Not clear |
| Hui Wu, Qizhu Tang, Jun Yang, Jiawang Ding, Ming Ye, Wusong Don. Atorvastatin ameliorates myocardial ischemia/reperfusion injury through attenuation of endoplasmic reticulum stress-induced apoptosis. International journal of clinical and experimental medicine. vol 7. issue 12. 2015-02-09. PMID:25663988. |
the expression levels of the ers proteins grp78, chop, caspase-12, c-jun nh2 terminal kinase (jnk) and phosphorylated jnk (p-jnk) were detected by western blot. |
2015-02-09 |
2023-08-13 |
rat |