| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Yining Lei, Manqin Li, Xinran Liu, Lu Zhang, Ruyi Zhang, Fei Ca. Nerolidol rescues hippocampal injury of diabetic rats through inhibiting NLRP3 inflammasome and regulation of MAPK/AKT pathway. BioFactors (Oxford, England). 2024-04-16. PMID:38624190. |
western blot was used to detect apoptosis (bcl-2, bax, and cleaved-caspase-3); synapses (postsynaptic densifying protein 95, syn1, and synaptophysin); mitochondria (drp1, opa1, mfn1, and mfn2); er (grp78, atf6, chop, and caspase-12); nlrp3 inflammasome (nlrp3, asc, and caspase-1); inflammatory cytokines (il-18, il-1β, and tnf-α); akt (p-akt); and mitogen-activated protein kinase (mapk) pathway (p-erk, p-p38, and p-jnk) related protein expression. |
2024-04-16 |
2024-04-18 |
rat |
| Hongzhu Zhang, Huimin Shi, Wan Xie, Meijuan Meng, Yan Wang, Nana Ma, Guangjun Chang, Xiangzhen She. Subacute ruminal acidosis induces pyroptosis via the mitophagy-mediated NLRP3 inflammasome activation in the livers of dairy cows fed a high-grain diet. Journal of dairy science. 2024-01-26. PMID:38278294. |
furthermore, western blot analysis showed that hg feeding led to increased expression of pyroptosis- and nlrp3 inflammasome-related proteins gsdmd n-terminal (gsdmd-nt; p = 0.006), il-1β (p < 0.001), il-18 (p = 0.076), cleaved-caspase-1 (p = 0.001), asc (p = 0.016), nlrp3 (p = 0.017), and cleaved-caspase-11 (p < 0.001) and upregulated expression of mitophagy-related proteins microtubule-associated protein 1 light chain 3 ii (map1lc3-ii; p = 0.001), beclin 1 (becn1; p = 0.001), parkin (p < 0.001), and pten induced kinase 1 (pink1; p = 0.006) in liver tissue. |
2024-01-26 |
2024-01-29 |
Not clear |
| Yulin Zhang, Hongxian Wu, Chaozhi Xu, Shanqian Li, Yue Hu, Zongyi Zhang, Guixian Wu, Yuling Liu, Lin Yang, Yue Huang, Wenjun Lu, Lina H. (-)-Epigallocatechin gallate alleviates chronic unpredictable mild stress-induced depressive symptoms in mice by regulating the mTOR autophagy pathway and inhibiting NLRP3 inflammasome activation. Food science & nutrition. vol 12. issue 1. 2024-01-25. PMID:38268911. |
then, elisa, western blot and qt-pcr tests were used to assess the expression of the key components of the nlrp3 inflammasome and its downstream inflammatory effectors (e.g., il-1β, il-18), autophagy markers (beclin-1, lc3, p62) and apoptosis markers (bax, bcl-2) in mouse brain tissues. |
2024-01-25 |
2024-01-27 |
mouse |
| Yunying Qian, Guiying Qian, Haiyan Ni, Danying Zhu, Weiqun Gu, Ximei Ca. Exploratory study on the mechanism of necrotic effect of nourishing cells in the context of genital tract infection in premature rupture of membranes. Medicine. vol 102. issue 50. 2023-12-20. PMID:38115314. |
western blot analysis was performed to measure the relative expression levels of cysteinyl aspartate specific proteinase-1 (caspase-1), cysteinyl aspartate specific proteinase-3 (caspase-3), nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3 (nlrp3), and interleukin (il)-1β proteins, which are associated with necrosis of placental nourishing cells, in the placenta of both groups. |
2023-12-20 |
2023-12-23 |
Not clear |
| Xiao-Juan Wang, Yu-Lu Wang, Nan Shao, Ting Ye, Shu Ye, Hua-Wu Gao, Yan Wan. [Huangdi Anxiao Capsules-containing serum protects cell model from cognitive dysfunction in diabetes via inhibiting NLRP3-mediated pyroptosis]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 48. issue 19. 2023-12-19. PMID:38114121. |
western blot was employed to determine the protein levels of postsynaptic density protein 95(psd-95), nlrp3, apoptosis-associated speck-like protein containing a card(asc), gasdermin d(gsdmd), gsdmd-n, and cleaved cysteinyl aspartate specific proteinase-1(caspase-1), and rt-pcr to determine the mrna levels of nlrp3, asc, gsdmd, and caspase-1. |
2023-12-19 |
2023-12-23 |
rat |
| Yongri Hong, Caichuan Wei, Miaoying Fu, Xinyang Li, Haiju Zhang, Baozhen Ya. MCC950 alleviates seizure severity and angiogenesis by inhibiting NLRP3/ IL-1β signaling pathway-mediated pyroptosis in mouse model of epilepsy. International immunopharmacology. vol 126. 2023-12-01. PMID:38039716. |
in addition, immunofluorescence staining, rt-qpcr, and western blot demonstrated that mcc950 inhibited the expression of the nlrp3 inflammasome and its related proteins in microglia, whereas microangiogenesis was found to be increased in the cerebral cortex and hippocampus of epileptic mice, and these effects could be reversed by mcc950. |
2023-12-01 |
2023-12-10 |
mouse |
| Meng Yuan, Asli F Ceylan, Rifeng Gao, Hong Zhu, Yingmei Zhang, Jun Re. Selective inhibition of the NLRP3 inflammasome protects against acute ethanol-induced cardiotoxicity in an FBXL2-dependent manner. Acta biochimica et biophysica Sinica. 2023-11-23. PMID:37994158. |
levels of nlrp3 inflammasome, mitophagy and apoptosis are evaluated by western blot analysis and immunofluorescence techniques. |
2023-11-23 |
2023-11-29 |
mouse |
| Fei Li, Hongxiang Zhu, Zijuan Chang, Ying L. Gentiopicroside alleviates acute myocardial infarction injury in rats by disrupting Nrf2/NLRP3 signaling. Experimental biology and medicine (Maywood, N.J.). 2023-10-18. PMID:37850391. |
reactive oxygen species (ros) and cell apoptosis were assessed, while nrf2 and the expression of the nlrp3 inflammatory body signal pathway were evaluated using western blot and immunofluorescence techniques. |
2023-10-18 |
2023-11-08 |
rat |
| Gehua Wen, Xiaoni Zhan, Xiaoming Xu, Xi Xia, Shukun Jiang, Xinghua Ren, Weishu Ren, Haoyang Lou, Lei Lu, Anca Hermenean, Jun Yao, Lina Gao, Baoman Li, Yan Lu, Xu W. Ketamine Improves the Glymphatic Pathway by Reducing the Pyroptosis of Hippocampal Astrocytes in the Chronic Unpredictable Mild Stress Model. Molecular neurobiology. 2023-10-15. PMID:37840071. |
meanwhile, ketamine reduced the level of hippocampus nlrp3 and the expression of its downstream molecules in chronic unpredictable mild stress (cums) mice model by western blot and reduced the colocalization of gfap and gsdmd by nearly 25% via immunofluorescent staining. |
2023-10-15 |
2023-11-08 |
mouse |
| Shuai Qiao, Xiangrui Zhang, Ziyi Chen, Ye Zhao, Chi-Meng Tzen. Alloferon-1 ameliorates estrogen deficiency-induced osteoporosis through dampening the NLRP3/caspase-1/IL-1β/IL-18 signaling pathway. International immunopharmacology. vol 124. issue Pt B. 2023-09-24. PMID:37742365. |
moreover, alloferon-1 suppressed the expression of the ovariectomy‑mediated inflammatory cytokines in the vertebrae bone tissues, including nucleotide-binding oligomerization domain-like-receptor family pyrin domain-containing 3 (nlrp3), cysteinyl aspartate specific proteinase-1 (caspase-1), interleukin 1β (il-1β) and interleukin 18 (il-18) which were determined by immunofluorescence staining and western blot. |
2023-09-24 |
2023-10-07 |
Not clear |
| Yousef Mohamadi, Maryam Borhani-Haghigh. TGN020 application against aquaporin 4 improved multiple sclerosis by inhibiting astrocytes, microglia, and NLRP3 inflammasome in a cuprizone mouse model. Journal of chemical neuroanatomy. 2023-07-02. PMID:37394105. |
astrogliosis, m1-m2 microglia polarization, nlrp3 inflammasome activation, and demyelination were investigated in the corpus callosum by immunohistochemistry, real-time pcr, western blot, and luxol fast blue staining. |
2023-07-02 |
2023-08-14 |
mouse |
| Jiaxuan Wu, Shengya Fang, Pengfei Feng, Chunjiang Cai, Leying Zhang, Ling Yan. Changes in expression levels of Nod-like receptors in the spleen of ewes. Animal reproduction. vol 20. issue 1. 2023-05-25. PMID:37228386. |
expression of nlr family, including nod1, nod2, class ii transactivator (ciita), nlr family apoptosis inhibitory protein (naip), nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 1 (nlrp1), nlrp3 and nlrp7, was analyzed using quantitative real-time pcr, western blot and immunohistochemistry analysis. |
2023-05-25 |
2023-08-14 |
Not clear |
| Lu Ji, Rong-Jie Jin, Lin L. Platelet-rich Plasma Improves Radiotherapy-induced Emotional Disorder and Cognitive Dysfunction, Neuroinflammation in Aged Rats by Inhibiting the Activation of NLRP3 Inflammasomes. Neurochemical research. 2023-04-12. PMID:37043084. |
besides, immunohistochemistry was used to detect the expression of microglial marker protein iba-1 in rat hippocampus; enzyme linked immunosorbent assay (elisa) to examine the levels of pro-inflammatory cytokines tumor necrosis factor-alpha (tnf-α), interleukin (il)-1 beta (il-1β), il-18, and monocyte chemoattractant protein 1 (mcp-1) in rat hippocampus; real-time quantitative reverse transcription pcr (qrt-pcr) and western blot to measure the levels of neurotrophic factors brain-derived neurotrophic factor (bdnf), tropomyosin-related kinase b receptor (trkb), and nerve growth factor (ngf) in rat hippocampus; and western blot also to observe the protein expression levels of nod-like receptor protein 3 (nlrp3), caspase-1, apoptosis-associated speck-like protein containing a card (asc), and il-1β in rat hippocampus. |
2023-04-12 |
2023-08-14 |
rat |
| Lijuan He, Wei Zhou, Lamei Wang, Na Tang, Yongjia Wang, Hua Zhong, Yan Tang, Dongmei Xi, Fang H. Murine Cytomegalovirus Infection Induced miR-1929-3p Down-Regulation Promotes the Proliferation and Apoptosis of Vascular Smooth Muscle Cells in Mice by Targeting Endothelin A Receptor and Downstream NLRP3 Activation Pathway. Molecular biotechnology. 2023-04-06. PMID:37022597. |
western blot detected proteins of cell proliferation, apoptosis, and the nlrp3 inflammasome. |
2023-04-06 |
2023-08-14 |
mouse |
| Siqi Liu, Chongyang Wang, Yulian Zhang, Yalin Zhang, Yilan Song, Jingzhi Jiang, Ruobai Liu, Hainan Jin, Guanghai Yan, Yongde Ji. Polydatin inhibits mitochondrial damage and mitochondrial ROS by promoting PINK1-Parkin-mediated mitophagy in allergic rhinitis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. vol 37. issue 4. 2023-03-11. PMID:36906289. |
the expressions of pink1, parkin, p62, lc3b, nlrp3 inflammasome proteins, and apoptosis proteins in nasal tissues and hnepcs were measured by western blot. |
2023-03-11 |
2023-08-14 |
mouse |
| Nevien Hendawy, Tala H Salaheldin, Sally A Abuelez. PCSK9 Inhibition Reduces Depressive like Behavior in CUMS-Exposed Rats: Highlights on HMGB1/RAGE/TLR4 Pathway, NLRP3 Inflammasome Complex and IDO-1. Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology. 2023-02-13. PMID:36781714. |
western blot and pcr analysis showed that alirocumab favorably modulated the hmgb1/rage/tlr4 axis, nuclear-factor-kappa-beta, nlrp3 inflammasome complex and ido-1 in the hippocampus of cums rats. |
2023-02-13 |
2023-08-14 |
rat |
| Nan Chen, Jie Deng, Zhihui Zhang, Xia Feng, Hongkang Wang, Jian Chen, Ling Li, Yemin Cao, Chenglin Jia, Yongbing Ca. Oxidative stress-triggered pyroptosis mediates Candida albicans susceptibility in diabetic foot. Microbial pathogenesis. 2022-09-10. PMID:36087690. |
notably, in this study, through the investigation of murine diabetic footpad c. albicans infection model and molecular biotechnology, including histopathological staining, immunofluorescence (if) staining, quantitative real-time pcr (qpcr), western blot (wb), flow cytometry (fcm), sandwich enzyme-linked immunosorbent assay (elisa) assays, we found that intense os responses in the footpad tissue not only mediated the activation of nf-κb protein complex, but also triggered downstream pyroptosis and apoptosis through nlrp3 inflammasome, which is one of the potential reasons for the severe condition of infectious skin injuries in diabetic mice. |
2022-09-10 |
2023-08-14 |
mouse |
| Jing Luo, Ning Jiang, Jialei Chen, Gao Yu, Jing Zhao, Changhong Yang, Yong Zha. Inhibition of miR-423-5p exerts neuroprotective effects in an experimental rat model of cerebral ischemia/reperfusion injury. Neuroscience. 2022-09-06. PMID:36067951. |
subsequently, western blot, elisa, mpo, tunel and commercial assay kits were applied to assess the influence of mir-423-5p on nlrp3 inflammasome, apoptosis, and oxidative stress levels in ischemic penumbra tissue. |
2022-09-06 |
2023-08-14 |
rat |
| Lijun Huang, Leiqiang Gong, Xueyan Huo, Lirong Lei, Qi Zhang, Yunjie Hu, Qixuan Kuang, Yu Gui, Yifei Dai, Yucheng Gu, Yun Deng, Dong Wang, Dale Gu. N-acetyldopamine dimer inhibits neuroinflammation through the TLR4/NF-κB and NLRP3/Caspase-1 pathways. Acta biochimica et biophysica Sinica. 2022-08-26. PMID:36017888. |
western blot analysis show that nadd inhibits the protein levels of toll-like receptor 4 (tlr4), nuclear factor kappa-b (nf-κb), nod-like receptor thermal protein domain associated protein 3 (nlrp3), asc and cysteinyl aspartate specific proteinase (caspase)-1, indicating that nadd may inhibit neuroinflammation through the tlr4/nf-κb and nlrp3/caspase-1 signaling pathways. |
2022-08-26 |
2023-08-14 |
Not clear |
| Li Cai, Zeng-Yu Yao, Lu Yang, Xiu-Hong Xu, Meng Luo, Miao-Miao Dong, Guo-Ping Zho. Mechanism of Electroacupuncture Against Cerebral Ischemia-Reperfusion Injury: Reducing Inflammatory Response and Cell Pyroptosis by Inhibiting NLRP3 and Caspase-1. Frontiers in molecular neuroscience. vol 15. 2022-05-23. PMID:35600074. |
ea was conducted at acupoints chize (lu5), hegu (li4), sanyinjiao (sp6), and zusanli (st36) for 1.5 h after cerebral ischemia-reperfusion injury for 20 min, and observation was carried out after 24 h. neurological deficit scores evaluated the neurological function, cerebral infarction volume was observed by triphenyl tetrazolium chloride (ttc) staining, hematoxylin and eosin (h&e) staining, tunel and caspase-1 double-labeled fluorescence staining, and nlrp3 and caspase-1 double-labeled immunofluorescence staining that were used to observe the morphology of neurons in hippocampus, and the protein expression of nlrp3, pro-caspase-1, cleaved caspase-1 p20, pro-interleukin-1β (il-1β), cleaved il-1β, and gsdmd was detected by western blot assay. |
2022-05-23 |
2023-08-13 |
mouse |