| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Yining Lei, Manqin Li, Xinran Liu, Lu Zhang, Ruyi Zhang, Fei Ca. Nerolidol rescues hippocampal injury of diabetic rats through inhibiting NLRP3 inflammasome and regulation of MAPK/AKT pathway. BioFactors (Oxford, England). 2024-04-16. PMID:38624190. |
western blot was used to detect apoptosis (bcl-2, bax, and cleaved-caspase-3); synapses (postsynaptic densifying protein 95, syn1, and synaptophysin); mitochondria (drp1, opa1, mfn1, and mfn2); er (grp78, atf6, chop, and caspase-12); nlrp3 inflammasome (nlrp3, asc, and caspase-1); inflammatory cytokines (il-18, il-1β, and tnf-α); akt (p-akt); and mitogen-activated protein kinase (mapk) pathway (p-erk, p-p38, and p-jnk) related protein expression. |
2024-04-16 |
2024-04-18 |
rat |
| Minfang Guo, Huiyu Zhang, Peijun Zhang, Jingwen Yu, Tao Meng, Suyao Li, Lijuan Song, Zhi Chai, Jiezhong Yu, Cungen M. [Knock-down of ROCK2 gene improves cognitive function and reduces neuronal apoptosis in AD mice by promoting mitochondrial fusion and inhibiting its division]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 39. issue 8. 2023-07-29. PMID:37515336. |
western blot analysis was used to detect the expression rock2, cleaved-caspase-3 (c-caspase-3), b-cell lymphoma 2 (bcl2), bcl2-related protein x (bax), p-drp1, mitochondrial fission 1 (fis1), optic atrophy 1 (opa1), mfn1 and mfn2. |
2023-07-29 |
2023-08-14 |
mouse |
| Yuan Luo, Pu Chen, Li-Ping Yang, Xiao-Hua Dua. [Quality control mechanism of mitochondria by 3,4-dihydroxybenzaldehyde through OGT-PINK1 pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 48. issue 12. 2023-06-29. PMID:37382015. |
therefore, western blot was employed to detect the expression of ogt, mitophagy-related proteins pink1 and e3 ubiquitin ligase(parkin), and mitochondrial kinetic proteins dynamin-like protein 1(drp1) and optic atrophy 1(opa1). |
2023-06-29 |
2023-08-14 |
rat |
| Xiaxia Xiong, Weiping Xiong, Linyuan Li, Juan DU, Daozhuang Wang, Xu Wan. [Down-regulation of miR-29a inhibits mitochondrial fusion and fission in rat H9c2 cardiomyocytes induced by oxygen and glucose deprivation/reoxygenation]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. vol 38. issue 11. 2022-11-03. PMID:36328430. |
western blot analysis was employed to test the levels of bcl2-associated x protein (bax), bcl2 antagonist/killer (bak), caspase-9, mitochondrial fission protein 1(fis1), mitofusin 1 (mfn1), mfn2, optic atrophy 1(opa1), phosphoryerated extracellular signal-regulated kinase (p-erk) and phosphoryerated dynamin related protein-1(p-drp1). |
2022-11-03 |
2023-08-14 |
rat |
| Nancy P Gómez-Crisóstomo, Selva Rivas-Arancibia, Erika Rodríguez-Martínez, Erick N De la Cruz-Hernández, Corazón de María Márquez Álvarez, Pablo Alexis Estrada Caraveo, Noemí Gelista Herrera, Marlen Valdés-Fuentes, Eduardo Martínez-Abundi. Chronic exposition to ozone induces cardiac antioxidant response and overexpression of either mitochondrial fision protein DRP1 and hipertrophyc-related proteins. Journal of bioenergetics and biomembranes. 2022-03-29. PMID:35347511. |
oxidative stress was evaluated by tbars and protein nitrosylation in addition to superoxide dismutase 1 (sod1) and catalase levels; the apoptosis related-proteins caspase 3, caspase 9, bax, bcl-2, and the mitochondrial dynamic-associated proteins fis1, drp1, opa1, and mfn1 were quantified by western blot among the cardiac hypertrophy indicator alpha-actin (cardiac actin). |
2022-03-29 |
2023-08-13 |
rat |
| Shunrong Zhang, Yue Gao, Jian'an Wan. Advanced glycation end products influence mitochondrial fusion-fission dynamics through RAGE in human aortic endothelial cells. International journal of clinical and experimental pathology. vol 10. issue 7. 2020-01-26. PMID:31966653. |
real time pcr method was used to detect the mrna expression levels of mitochondrial dynamics regulatory genes mitofusin 1 (mfn1), mitofusin 2 (mfn2), optic atrophy 1 (opa1), and dynamin-related protein 1 (drp1) of haecs, and western blot method was used to detect the protein expression levels of these regulatory genes. |
2020-01-26 |
2023-08-13 |
human |
| Hong-Wei Wang, Shi-Quan Zhu, Jing Liu, Cheng-Yi Miao, Yan Zhang, Bian-Hua Zho. Fluoride-induced renal dysfunction via respiratory chain complex abnormal expression and fusion elevation in mice. Chemosphere. vol 238. 2020-01-01. PMID:31524603. |
the expression of mitochondrial fusion protein including mitofusin-1 (mfn1) and optic atrophy 1 (opa1), and mitochondrial respiratory chain complex subunits, including ndufv2, sdha, cyc1 and cox Ⅳ, were detected via real-time polymerase chain reaction, immunohistochemistry staining and western blot, respectively. |
2020-01-01 |
2023-08-13 |
mouse |
| Ye-hao Zhang, Wei-hong Cong, Li Xu, Bin Yang, Ming-jiang Yao, Wen-ting Song, Jian-xun Li. [Effect of Sailuotong capsule on mitochondrial dynamics in focal cerebral ischemia/reperfusion rats]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. vol 40. issue 10. 2015-10-27. PMID:26390660. |
the expressions of dynamin-related protein 1 ( drp1) and optic atrophy 1 (opa1) were tested by western blot. |
2015-10-27 |
2023-08-13 |
rat |
| D Nguyen, M V Alavi, K-Y Kim, T Kang, R T Scott, Y H Noh, J D Lindsey, B Wissinger, M H Ellisman, R N Weinreb, G A Perkins, W-K J. A new vicious cycle involving glutamate excitotoxicity, oxidative stress and mitochondrial dynamics. Cell death & disease. vol 2. 2012-03-16. PMID:22158479. |
expression of nmda receptors (nr1, 2a, and 2b) in the retina of opa1(enu/+) mice was significantly increased as determined by western blot and immunohistochemistry. |
2012-03-16 |
2023-08-12 |
mouse |
| An-Guor Wang, Ming-Ji Fann, Hsin-Yi Yu, May-Yung Ye. OPA1 expression in the human retina and optic nerve. Experimental eye research. vol 83. issue 5. 2006-12-22. PMID:16857190. |
western blot and immunofluorescence staining were performed to examine opa1 expression in the human retina and optic nerve. |
2006-12-22 |
2023-08-12 |
human |
| Stefanie Bette, Holger Schlaszus, Bernd Wissinger, Richard Meyermann, Michel Mittelbron. OPA1, associated with autosomal dominant optic atrophy, is widely expressed in the human brain. Acta neuropathologica. vol 109. issue 4. 2005-09-01. PMID:15700187. |
northern blot analyses showed that opa1 is expressed in all tissues examined, with the highest transcript level in the retina and in the brain. |
2005-09-01 |
2023-08-12 |
human |