| Publication |
Sentence |
Publish Date |
Extraction Date |
Species |
| Marta Pietraszek, Grażyna Greczka, Anna Bartochowska, Wojciech Gawęck. Polish version of the COMQ-12 questionnaire, a new validated tool for the assessment of the quality of life in patients with chronic otitis media. Otolaryngologia polska = The Polish otolaryngology. vol 78. issue 2. 2024-04-16. PMID:38623854. |
the internal consistency, reliability, and construct validity of the questionnaire were analyzed using cronbach's alpha and mcdonald's omega coefficients, spearman's rho correlation coefficient, and the mann-whitney test, respectively.</br> <b><br>results:</b> high internal consistency, reliability, and construct validity of the polish version of the comq-12 questionnaire were shown in the course of statistical analysis. |
2024-04-16 |
2024-04-18 |
human |
| Beata Jastrzebska, Tivadar Orban, Marcin Golczak, Andreas Engel, Krzysztof Palczewsk. Asymmetry of the rhodopsin dimer in complex with transducin. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. vol 27. issue 4. 2013-06-17. PMID:23303210. |
thus, our study shows that each of the monomers contributes unequally to the pentameric (2:1:1:1) complex of rho dimer and gt heterotrimer, validating the oligomeric structure of the complex and the asymmetry of the gpcr dimer, and revealing its structural/functional signature. |
2013-06-17 |
2023-08-12 |
cattle |
| Beata Jastrzebska, Anna Goc, Marcin Golczak, Krzysztof Palczewsk. Phospholipids are needed for the proper formation, stability, and function of the photoactivated rhodopsin-transducin complex. Biochemistry. vol 48. issue 23. 2009-07-30. PMID:19413332. |
herein, we report isolation conditions that enhance the stability and preserve the activity and proper stoichiometry of productive complexes between the purified prototypical gpcr, rhodopsin (rho), and the rod cell-specific g protein, transducin (gt). |
2009-07-30 |
2023-08-12 |
cattle |
| Beata Jastrzebska, Anna Goc, Marcin Golczak, Krzysztof Palczewsk. Phospholipids are needed for the proper formation, stability, and function of the photoactivated rhodopsin-transducin complex. Biochemistry. vol 48. issue 23. 2009-07-30. PMID:19413332. |
these results strongly indicate a lipid requirement for stable complex formation in which the likely oligomeric structure of rho provides a superior platform for coupling to gt, and phospholipids likely form a matrix to which gt can anchor through its myristoyl and farnesyl groups. |
2009-07-30 |
2023-08-12 |
cattle |
| Anna Goc, Thomas E Angel, Beata Jastrzebska, Benlian Wang, Patrick L Wintrode, Krzysztof Palczewsk. Different properties of the native and reconstituted heterotrimeric G protein transducin. Biochemistry. vol 47. issue 47. 2009-03-16. PMID:18975915. |
here we describe a new method for gt purification from native bovine rod photoreceptor membranes without subunit dissociation caused by exposure to photoactivated rhodopsin (rho*). |
2009-03-16 |
2023-08-12 |
cattle |
| B M Willardson, B Pou, T Yoshida, M W Bitensk. Cooperative binding of the retinal rod G-protein, transducin, to light-activated rhodopsin. The Journal of biological chemistry. vol 268. issue 9. 1993-04-22. PMID:8454608. |
the gt alpha subunit in the absence of gt beta gamma also bound rho* in a cooperative manner. |
1993-04-22 |
2023-08-12 |
Not clear |
| B M Willardson, B Pou, T Yoshida, M W Bitensk. Cooperative binding of the retinal rod G-protein, transducin, to light-activated rhodopsin. The Journal of biological chemistry. vol 268. issue 9. 1993-04-22. PMID:8454608. |
cooperativity was assessed by measuring the association of 125i-labeled transducin (gt) to rho* in urea-stripped rod outer segment membranes at equilibrium. |
1993-04-22 |
2023-08-12 |
Not clear |
| Arshavsky VYu, M P Gray-Keller, M D Bownd. cGMP suppresses GTPase activity of a portion of transducin equimolar to phosphodiesterase in frog rod outer segments. Light-induced cGMP decreases as a putative feedback mechanism of the photoresponse. The Journal of biological chemistry. vol 266. issue 28. 1991-11-08. PMID:1655754. |
here we report that there are two portions of gt in frog rod outer segments (ros) expressing different rates of gtp hydrolysis: 19.5 +/- 3 mmol of gt/mol of rho, equivalent to that amount which participates in pde activation, hydrolyzing gtp at a rate of approximately 0.6 turnover/s ("fast") and the remaining gt (80.5 +/- 3 mmol/mol rho) hydrolyzing gtp at a rate of 0.058 +/- 0.009 turnover/s. |
1991-11-08 |
2023-08-11 |
Not clear |
| Arshavsky VYu, M P Gray-Keller, M D Bownd. cGMP suppresses GTPase activity of a portion of transducin equimolar to phosphodiesterase in frog rod outer segments. Light-induced cGMP decreases as a putative feedback mechanism of the photoresponse. The Journal of biological chemistry. vol 266. issue 28. 1991-11-08. PMID:1655754. |
in rod photoreceptor cells, the light response is triggered by an enzymatic cascade that causes cgmp levels to fall: excited rhodopsin (rho*)----rod g-protein (transducin, gt)----cgmp-phosphodiesterase (pde). |
1991-11-08 |
2023-08-11 |
Not clear |